Solid phase microextraction quotation

Thin film solid-phase microextraction(Thin Film SPME)， TF-SPME, abbreviated as TF-SPME, is a new extraction tool developed by Professor Janusz Pawliszyn of the University of Waterloo and Academician of SPME. It is achieved by coating the adsorption phase (extraction layer) onto a carbon mesh.

This technology, like SPME and SBSE, is a green solvent-free extraction and concentration technique suitable for analyzing trace volatile organic compounds (VOCs, SVOCs). By increasing the surface area and volume of the adsorption phase of TF-SPME, better extraction performance was achieved for polar compounds and volatile organic compounds VVOCs, with the advantage of shorter extraction time.

There are multiple adsorption phases available, including PDMS/DVB, PDMS/Carboxen, and HLB/PDMS. It can be used alone or at the same time with SBSE, and is suitable for food, beverage, essence and spice, environmental testing and other industries. The design of its thin film is more suitable for contact sampling, such as skin and material surfaces.

TF-SPME can be used for headspace or immersion extraction

Advantages of TF-SPME

The large surface area of TF-SPME improves sampling efficiency and helps reduce the time required to reach equilibrium. The surface area of TF-SPME membrane increased by 20 times compared to the surface area of 100 µ m SPME fiber. For the extraction of several polycyclic aromatic hydrocarbons (PAHs), the extraction yield of TF-SPME is 7-20 times higher than that of SPME (Bruheim, Liu,&Pawliszyn, 2003).

Overview of Extraction Phase Surface Area and Volume of Various Adsorption Extraction Devices

Coating of TF-SPME

TF-SPME Sampling and Analysis Process

TF-SPME application

Environment/Water

TF-SPME technology is particularly suitable for on-site sampling of environmental substrates, especially in water systems. TF-SPME can perform rapid on-site sampling of environmental samples and avoid loss and degradation of analytes during collection, transportation, and storage. In addition to using desktop GC-MS analysis, portable GC-MS can also be used for analysis. TF-SPME has been successfully used as a passive sampler for on-site water quality testing in different water systems to determine polycyclic aromatic hydrocarbons (Bragg et al., 2006); Qin et al. (2008) and industrial impact on water to determine pollutants such as toluene, ethylbenzene, and xylene (Grandy, J. J., Boyaci, E.,&Pawliszyn, J., 2016). Grandi et al. (2018) used HLB/PDMS coating for on-site extraction of chlorinated analytes from private hot tubs. HLB/PDMS coating is also applied to produced water (PW), which is the main waste byproduct of hydraulic fracturing.

Food, flavor, essence, spice and beverage

The quality, authenticity, and safety of food and beverage products need to be constantly monitored. The aroma and flavor characteristics of these products are extremely important for customer acceptance, and identifying odor components can help identify product defects. TF-SPME and its combined extraction with TF-SPME and Twister have been used to analyze various food and beverage matrices, and their response to various compounds has been improved compared to SPME or SBSE alone. In direct immersion mode, TF-SPME with DVB/PDMS coating can efficiently extract important volatile aromatic compounds from many beverages, including wine, coffee, and soda water. The comparison between traditional SPME and TF-SPME (DVB/PDMS) for extracting white wine (everlasting longing for each other/Qiongyao Syrup mixture) showed that TF-SPME had significantly higher response to all identified compounds.

Total ion chromatogram of everlasting longing for each other/Qiongyao Syrup mixed liquor and stack view of identified compounds using SPME (top) and TF-SPME (bottom). (GERSTEL Application Literature 200)

Odor analysis

One of the tasks of analyzing materials and consumer goods is to evaluate and identify odors. Odors in materials may be a major issue for manufacturers as they can lead to consumer complaints and brand damage. The compounds that cause these odors typically exist in trace amounts, but due to the low odor threshold, they can still be detected by the nose, making their identification in complex matrices complex. GERSTEL application literature 218 uses gas chromatography sniffing techniques GC-O-MS and TF-SPME for sample extraction and identification of odor compounds in paper products. This technology can identify trimethylamine as one of the main compounds causing the unpleasant fishy smell detected in paper products.

Comparison of the results of using individual and combined extraction techniques TF-SPME and SBSE-TD-GC-MS to determine aroma compounds in whiskey.

Twister and TF-SPME combined extraction, GERSTEL thermal desorption unit (TDU) thermal desorption, and large volume cold injection system (CIS) were used to complete thermal injection, and the analyte was introduced into GC.