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E-mail
pumei@pumei17.com
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Phone
18621660048
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Address
No. 629 Changzhong Road, Fengxian District, Shanghai
Shanghai Pumei Instrument Technology Co., Ltd
pumei@pumei17.com
18621660048
No. 629 Changzhong Road, Fengxian District, Shanghai
Gas chromatograph for detecting alcohol (ethanol) content in blood
Shanghai PumeiGas chromatograph for detecting alcohol (ethanol) content in blood
Reference standardsGA/T842-2019
This method is applicable for qualitative and quantitative analysis of alcohol (hereinafter referred to as ethanol) in the blood of individuals during road traffic activities.
This method utilizes the volatility of ethanol and uses tert butanol as the internal standardGas chromatography combined with headspace samplerThe injection technique adoptsHeadspace gas chromatography methodWe obtained satisfactory analysis results. This method is characterized by simplicity, speed, high sensitivity, good reproducibility, and the ability to achieve semi automation. Use a 2120 hydrogen flame ionization detector for detection; By comparing with ethanol standards operated in parallel, qualitative analysis was conducted based on retention time, and quantitative analysis was performed using the internal standard method based on the peak area ratio of ethanol to the internal standard.
Alcohol content standard: The alcohol concentration indicated on the bottle (only taking numbers) multiplied by the amount of alcohol (measured in liters) equals the unit of alcohol (approximately).
For example, listening to 330 milliliters of beer with a 5% alcohol concentration, there are approximately 1.65 units of alcohol in 5 x 0.33; A 750ml bottle of red wine with a 12% alcohol concentration contains approximately 9 pieces of 12 x 0.75
Blood alcohol content standard: Alcohol intake calculation formula: Alcohol intake (grams)=Alcohol consumption (milliliters) x Alcohol concentration (%) x 0.8 (Alcohol density)
Blood alcohol content standard: When a person drinks alcohol, the alcohol is absorbed but not digested. A portion of the alcohol evaporates, passes through the alveoli, and is exhaled out of the body.
After measurement, the ratio of alcohol concentration in exhaled breath to alcohol concentration in blood is 2100:1, which means that the amount of alcohol contained in every 2100 ml of exhaled breath is equal to the amount of alcohol contained in 1 ml of blood. A simple alcohol detector utilizes the principle that there is a certain proportional relationship between alcohol concentration and blood alcohol concentration. By measuring the driver's exhaled breath, the alcohol content in the subject's blood can be quickly calculated.
Operating temperature: 200 ℃
Standard concentration: 0.107mg/ml
Sample temperature: 110 ℃
Detector: FID
Quantitative method: External standard method
Column temperature: 120 ℃
Injection I: 180 ℃
Pressurization pressure: 0.16MPa
Carrier gas pressure: 0.05Mpa
Sample bottle: 80 ℃
Valve temperature: 100 ℃
Pipeline: 80 ℃
configuration
1Instrument namegas chromatography
Host: Hydrogen flame detector, packed column and capillary injection system, programmed temperature rise, intelligent rear door opening system.
Automatic headspace sampler,
Sealing pliers (1 set), headspace bottle, cap, and pad (100 sets).
2 chromatographic columns: selected according to the requirements of the sample
3 Chromatography Workstation Dual Channel External
4 computers, printers